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Another issue with roads is that they divide up animal populations since many won’t cross roads at all, making the populations less genetically diverse. The book, Traffication delves into this topic.
I started my working life as a Trainee Surveyor back in the 80s and 90s with The Main Roads Dept of Qld. I walked 1000s of kms of roads doing surveys, and encountered many many thousands of dead animals, not just on the road, but the verge, the table drains etc. It was a constant stench of dead animal as I walked and cars roared past…
They greatest ecological crime we have ever commited as a species is the road.